Hymenoptera

Internal nodes are labeled as: <Node ID>_Bootstrap_Concordance
Summary
# species24
# single-copy orthologs2124
Fossil calibrationsNode 25 at 226.4-411mya
Node HY25 at 89.9-93.9mya
Node HY13 at 23-28.4mya
Contentious nodesHY3 clade
# gene families for CAFE19514
CAFE estimated lambda0.0009
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Methods (brief)

Orthogroups were obtained from OrthoDBv8. Single copy sequences were aligned with PASTA and RAxML was used to make gene trees. ASTRAL was used to estimate the species tree topology from gene trees while RAxML on a concatenated alignment was used to estimate branch lengths in terms of relative substitutions. r8s was used with the fossil calibrations to get branch lengths in terms of millions of years. This species tree was then used with CAFE and Dupliphy to reconstruct ancestral gene counts and gains and losses were assessed.